Imaging technologies are widespread in the life science laboratories today. Many biological questions may be addressed applying fluorescence microscopy and live imaging technologies. Advances in the available instruments and technologies are continuously evolving. The course will examine some of the cutting-edge technologies available today, through realworld examples provided by our panel of experts and with our co-sponsor Nikon will allow the setup of four imaging/analysis stations: confocal imaging, spinning disk imaging, time-lapse microscopy, image processing and quantitative analysis. To this end morning theoretical sessions will provide the necessary background and afternoon practical sessions will permit hands-on experience on state-of-the-art instruments.. It is expected that the participants at the end of the Course will be capable of applying fluorescence microscopy and live cell imaging to their experimental needs.

• October 16 2017 Bright Field and Fluorescence Microscopy: basic principles Valentina Russo – Angelo Balsamo 10.30 – 12.30 Microscopy Working Stations Hands-on 14.00 – 17.00

• October 17 2017 Live Cells Microscopy: Imaging and Time-Lapse Inverted Microscope: basic principles Pietro Cirigliano – Angelo Balsamo 10.30 – 12.30 Imaging solution Pietro Cirigliano – Angelo Balsamo 14.00 – 17.00

• October 18 2017 Live Cells Microscopy: Imaging and Time-Lapse Microscopy Working Stations Hands-on Pietro Cirigliano 9.00 – 12.30

• October 19 2017 Setup and technology of a Confocal Microscope Giacomo Cozzi 10.30 – 12.30 Microscopy Working Stations Hands-on Giacomo Cozzi 14.00 – 17.00

• October 20 2017 Setup and technology of a Confocal Microscope Microscopy Working Stations Hands-on Giacomo Cozzi 9.00 – 12.30